Genolyte® – nieuwe multifunctionele homogeniser & cellyser

Deze multifunctionele homogeniser & cellyser van SPEX Europe  is ideaal voor het homogeniseren van een klein aantal monsters in vials (6 x 2mL, 4 x 5mL, 2 x 7mL en 1 x 12mL).

De Genolyte maakt een oscillerende beweging met een hoge intensiteit. Deze beweging in combinatie met een snelheid, die instelbaar is tussen de 750 en 4000 RPM, zorgen voor een zeer effectieve homogenisatie. En dat binnen enkele seconden!

Het bijzondere van deze homogeniser is dat die zowel ingezet kan worden voor de monstervoorbereiding van DNA/RNA- en eiwitextracties alsook voor het verpulveren van de hardste steensoorten tot fijn poeder.

Geschikt voor de volgende monstertypen:

dierlijk en plantaardig weefsel, celculturen, bacteriën, gist, ontlasting, aardemateriaal, zaden, dierlijk weefsel, bacteriën, gist, ontlasting en aarde

…en dus ook het verpulveren van de hardste steensoorten, mineralen, cement en keramiek.

Is your spectrophotometer compiant with the latest USP and EP Chapters?

New editions of United States General Pharmacopeia Chapter <857> and European Pharmacopoeia Chapter 2.2.25, giving guidance on instrument qualification for ultraviolet and visible spectrophotometry, have recently been published. The former becomes mandatory on 1st December 2019 and the latter on 1st January 2020. Both introduce new approaches to qualification and suggest a variety of new reference materials for qualification measurements. While there are several areas of conformity between the two new standards, there are also some important differences.

What do they have in common?

Earlier versions of both standards contained a fairly limited set of tests to control an instrument’s performance for wavelength, absorbance, stray light and resolution (spectral bandwidth). Provided an instrument passed these tests it could be claimed that it was ‘pharmacopoeia compliant’. The limitation of this approach is that a qualification carried out under one set of operating conditions might not be valid under another set. A simple example would be a qualification carried out in the visible using one light source when the actual analysis was to be carried out in the UV region using a different source. While the same parameters must still be qualified, the requirement is now to demonstrate ‘fitness for purpose’, namely that the instrument has the performance to undertake the actual analysis to the required accuracy and precision. The user must therefore determine, for these four parameters, the range over which the system will be used in the actual analysis and prove compliance over that range. One consequence of this is that the simplistic approach often adopted in the past – one qualification test for each of the four parameters – may not suffice. Both standards now recommend, directly or by implication, that the values of the references used for qualification should ‘bracket’ the values to be used in the proposed analysis, which may mean that more than one reference is required. There is also a specific new requirement, in both standards, to qualify absorbance linearity: this will almost certainly require the use of more than one absorbance reference. To help meet these new requirements, additional reference materials are cited in both standards. Both standards also allow – the USP strongly advocates – the use of commercially available Certified Reference Materials.

How do they differ?

The major difference between the two new standards is their scope. Whereas USP <857> is limited to UV-visible spectrophotometers as described in USP <1857>, the new EP standard is extended to encompass HPLC UV detectors and PAT (process analytical technology) applications. This is a considerable divergence – USP <857> specifi cally excludes HPLC detectors from its scope whereas EP 2.2.25 will be mandatory for HPLC detectors. The following notes relate to spectrophotometers: HPLC is covered separately.

Another major difference is in the approach to the test materials to be used for qualifi cation. The USP states: ‘Whenever possible, certifi ed reference materials (CRMs) are to be used in preference to laboratory-prepared solutions’ and now lists a greater variety of commercially available CRMs to make it easier to establish ‘fi tness for purpose’ over a range of operating parameters. The EP, conversely, gives details for the preparation of relatively few test solutions, one of which (caffeine) is not available commercially as a CRM. Fortunately, however, it still allows the use of Certifi ed Reference Materials, so compliance can still be achieved without the need to prepare solutions in the laboratory.

Will I need new reference materials?

Most laboratories working in a regulated environment will already have a selection of references for instrument qualification. To determine if any additional references are needed to meet the new regulations the wavelength and absorbance values expected in the proposed analyses should be checked to see if they are encompassed by the available references. If not, additional references will be required. For example, holmium oxide solution is the most widely used wavelength reference, with 14 peaks covering wavelengths from 240 nm to 650 nm. Provided the wavelengths to be used for analysis lie within these limits, no additional wavelength references should be required. For wavelengths below 240 nm, however, both standards now recommend cerium oxide solution, covering 200 nm to 270 nm. For even lower wavelengths, a ‘Deep UV’ CRM is available from Starna Scientific (Hainault UK), with certified wavelength and absorbance values down to 191 nm. When including analysis above 650 nm, didymium oxide is frequently used.

For absorbance qualification, the new linearity specifications mean that more than one reference will almost certainly be required: the USP recommends that linearity is controlled at a minimum of three absorbance levels over the expected range. Potassium dichromate solution has been used for many years and covers wavelengths from 235 to 350 nm. For lower wavelengths, nicotinic acid is now recommended, covering 210-270 nm. Both are commercially available as CRMs, at a range of concentrations with absorbance values up to 3A and can be purchased in convenient ‘linearity sets’. For the visible region, the USP now lists metal-on-quartz filters; while not compatible with all instruments, these filters can be used over a wide wavelength range (250 to 850 nm). For the visible region, wellestablished neutral density (grey glass) filters are available.

Stray light qualification: whereas EP 2.2.25 formerly named just one stray light reference, potassium chloride solution, both standards now list several references covering wavelengths from 190 nm to 400 nm. Note that USP <857> now permits the use of the traditional “specified wavelength” method as well as the ‘filter ratio’ or Mielenz method cited in the 2015 edition.

Both standards recommend the well-established toluene-in-hexane solution for resolution (bandwidth) qualification.

Other than the references named in the standards, a wide range of CRMs for spectrophotometry is available (see table below):

Wavelengh range of Certified Reference Materials

HPLC and PAT

The inclusion of these applications of UV-visible spectrophotometry in EP 2.2.25 requires different qualification
procedures from conventional spectrophotometry. All the references discussed above are available either as sealed
solution cells or solid filters and therefore involve no manipulation other than insertion in the instrument’s sample
holder. A few HPLC detectors can accept a conventional solution cell or filter, but for others and for PAT applications, it will be necessary to physically transfer the test solution into the measurement cell. Three of the references mentioned above, namely cerium oxide, holmium oxide and nicotinic acid solutions, are available as CRMs in vial format for this purpose and were developed specifically for HPLC. A fourth, Starna Green, can be used for both wavelength and absorbance qualification and is a specially formulated dye mixture that is
very stable, non-toxic and REACH compliant.

Conclusions

The new editions of USP <857> and EP 2.2.25 take a more holistic approach to the qualifi cation of UV-visible spectrophotometers, which must now be performed under conditions that approximate as closely as possible to those to be used for analysis. While some aspects of the new regulations may appear contradictory, the judicious selection of Certifi ed Reference Materials will allow instrument users to comply with the new regulations.

Credits for this article go to Nathan Hulme, Starna Scientific Ltd, who wrote this article. It is originally published on www.labmate-online.com.

If you have any questions about standards for your spectrophotometer or would like a quote, please call us on +31 (0) 88 46 78 786 or complete this form.

 

 

 

Status deliveries and service in relation to Corona virus

Dear customer,

Because of the coronavirus outbreak we live in a time with many questions.

It affects us all. More than ever before, it is important for your organisation to be able to guarantee continuity as much as possible, so that your contribution to our economy does not disappear.

We remain at your service, you can rely on the complete team of Instrument Solutions.

Supplies of instrument or consumables

To date, our foreign suppliers have not yet caused us any delays or that shipments are being temporarily detained.

However, this situation may change at any time. We advise you to build up sufficient stock so that you do not run into problems in the event of possible lockdowns. If you need an overview of products you order from us, we will be happy to send you this.

It concerns for instance products of the following brands: Inorganic Ventures – SCP Science – Glass Expansion – SPEX – Savillex – Macherey Nagel – LABC – Starna – CTC – Scion.  A complete overview of brands can be found here.

Technical Service

We will do everything in our power to continue to assist you in the event of technical malfunctions. Of course the safety of you and our Service & Product Specialists comes first. This means that if it is safe, we can carry out work at your location. If it is not safe or allowed, we can assist you remotely to solve the malfunctions of your lab equipment together. If you have a service request, you can let us know via a Service Ticket.

If you already have a General Service Agreement, you will be given priority.

Sales

Where possible, visits can be made remotely. We have possibilities for video meetings, presentations and product demonstrations, easy to give on your PC, tablet or smartphone. In this way, projects are minimally delayed.

If you need a call, please call us at +31 88 467 87 86.

We wish you a lot of strength in these turbulent times.

Meet us at Laborama 2020 (new dates)

Instrument Solutions participates with a booth at Laborama Expo on 11 and 12 June 2020 in Brussels. Originally this event was planned to take place on March 19 and 20, but due to the Corona virus it has been postponed.

For the Belgian market, this annual Expo is the meeting place for the laboratory sector.

Where is it? Brussels Expo – Palace 1 – Belgium

Meet us at booth number E20.

What you can expect at our stand:

Silent compact Air Compressor AirComp200

Claind, Italian manufacturer of gas generators since 1979, has launched a new compact air compressor. It has the power of an industrial compressor, but made it suitable for the laboratory.

The compressor delivers 180 Nl/minute of ultra dry oil-free compressed air suitable for feeding all nitrogen generators. Also suitable for general use.

For the lab a low noise level is important and the AirComp 200 is therefore quiet (< 58 dB). For years Claind has identified the most important wishes of laboratory users and integrated these into the AirComp 200. This makes the AirComp 200 unique!

Lowest noise level in the market

A pleasantly compact, aesthetic compressor that can be placed under the lab table. Ideal for the laboratory.

The AirComp 200 has a high production capacity (180 Nl/minute at 8.5 bar). Depending on the desired flow, the speed is automatically controlled so that in most situations only a small part of the capacity is used. The effect of this is:

  • a low electricity consumption;
  • Reduced engine load resulting in longer compressor life;
  • that it is low-maintenance thanks to the variable speed and low temperature that is released.

A built-in membrane dryer provides ultra dry air (Outlet Dew Point -60°C).

Lastly, the AirComp 200 is remotely controllable from the PC or smartphone and Operating parameters can be monitored in real time.

 

Specifications
Flow Rate180Nl/min (210 l/min FAD) @ 7 bar
Technologies employedOil free SCROLL compressor with Universal Power Supply and Electronic Speed Control
Output pressure6 ÷ 9,5 bar
Air output characteristicsParticulate = 0,01 mmm, Moisture=  -60°C dew point ATP @ 9,5bar 50Nl/min
Electrical Supply230 Vac ± 10%, 50/60, Hz 2,5kW
Index of protectionIP20
Noise Minimum: 54 dBA    Medium: 58 dBA
Operating temperature5°C – 40°C
Environmental HumidityMax 90% without condensation
Max AltitudeTested up to 2000 m from the sea level
DimensionsHeight: 79,5cm, Width: 68cm,  Depth: 83cm, Weight: 148kg
Pneumatic ConnectionsPurified Air Output: G 1/2” female

 

 

 

Freezer Mill ideal for modified RoHS regulations

The RoHS Directive is an EU directive for the restriction of hazardous substances in electronic products. RoHS is an abbreviation of Restriction of Hazardous Substances. Until recently, this directive listed 6 heavy metals that could be restricted in products by manufacturers of these devices. Four phthalates have now been added to these heavy metals for the RoHS3 version.

For years the Freezer/Mill of manufacturer SPEX SamplePrep has been grinding electronic & electrical components (for heavy metals) and plastic children’s toys & care products (for phthalates) into a homogeneous fine powder. This fine powder ensures efficient extraction of residues at low level and is therefore an ideal device to prepare samples for further downstream analysis. The Freezer/Mill therefore plays an important role in demonstrating whether the individual components and end product are produced in accordance with RoHS regulations.

The EU RoHS3 specifies maximum levels for the following 10 restricted substances:

  • Cadmium (Cd): < 100 ppm
  • Lead (Pb): < 1000 ppm
  • Mercury (Hg): < 1000 ppm
  • Hexavalent chromium: (Cr VI) < 1000 ppm
  • Polybrominated biphenyls (PBB): < 1000 ppm
  • Polybrominated diphenyl ethers (PBDE): < 1000 ppm
  • Bis(2-Ethylhexyl) phthalate (DEHP): < 1000 ppm
  • Benzyl butyl phthalate (BBP): < 1000 ppm
  • Dibutyl phthalate (DBP): < 1000 ppm
  • Diisobutyl phthalate (DIBP): < 1000 ppm

The first six were already applicable to the original RoHS, while the last four were added under RoHS 3, which came into force on 22 July 2019.

In order to comply with these new regulations, companies and local authorities will have to test products to ensure compliance.

Waste from Electrical and Electronic Equipment

Related to RoHS is the WEEE, which stands for Waste from Electrical and Electronic Equipment. The WEEE Directive 2002/96/EC requires the treatment, recovery and recycling of electrical and electronic equipment (90% goes to landfill). All electronic products on the EU markets must comply with the WEEE standard and carry the “Wheelie Bin” sticker.

Functioning Freezer/Mill:

The sample is placed in a special fully sealable Grinding Vial and immersed in liquid nitrogen to reach cryogenic temperatures. The sample is then pulverized by a stainless steel impacter which is magnetically beaten back and forth against two stationary end plugs of the vial by induction. Because the vial is immersed in liquid nitrogen throughout the grinding cycle and is tightly sealed, it retains hazardous and volatile substances and does not cross-contaminate other samples.

Special chromium-free vial sets are available for analysis of the elements. (Of course no Hg, Cd, or Pb is present in these sets either)

New – HydraMist Spray Chamber

Improve measurement sensitivity of elements such as Sb, Se, Tl and Hg by a factor of 10

The HydraMist of Glass-Expansion is a sensitive, simple-to-use spray chamber for Inductively Coupled Plasma (ICP) that allows simultaneous operation of both conventional pneumatic nebulization and cold-vapor/hydride generation. Cold vapor generation can provide more than 10-fold improvement in sensitivity on ICP for cold vapor forming elements such As, Sb, Se, Tl and Hg. The generation of volatile species of these elements results in increased analyte loading of the analytical plasma giving lower detection limits.

Cold vapor generation can provide more than 10-fold improvement in sensitivity on ICP for cold vapor forming elements such As, Sb, Se, Tl and Hg.

Design

Its design is based upon Glass Expansion’s industry-standard cyclonic 

spray chamber, giving excellent sensitivity and short-term analytical precision with fast washout. It features a secondary inlet port that mixes the aerosolized sample and liquid reductant inside the spray chamber for rapid conversion of the As, Sb, Se, Tl and Hg analytes into volatile hydride species. A unique drain design ensures fast, complete removal of waste from the spray chamber, eliminating excess hydrogen build-up that causes sample reflux degrading analytical precision.

HydraMist spray chamber features:

  • Same outstanding short-term analytical precision and washout as other Glass Expansion cyclonic spray chambers
  • Fast and complete vapor phase formation of volatile As, Se, Sb, Tl and Hg species for the best detection limits in hydride generation mode
  • A unique drain design to eliminate hydrogen build-up and sample reflux that degrades short-term precision
  • Economic, just replace your current spray chamber and keep your existing nebulizer
  • Improve productivity by analysing non-hydride forming elements and cold vapor elements simultaneously, avoiding system shutdowns to change over between the hydride generator accessory and conventional pneumatic nebulization

Simple Mode

With a simple to setup with fast changeover, the HydraMist Spray chamber can be operated in one of 3 different modes. The simple mode provides (figure 1.) simultaneous hydride and pneumatic nebulization mode with 5 fold improvements for detection limits of the hydride forming elements, while maintaining analytical performance for non-hydride elements.

Sensitive Mode

With the addition of an extra sample input and t-piece (figure 2.) the HyrdraMist provides a sensitive, simultaneous cold vapor and pneumatic nebulization mode with more than 10 fold improvements in detection limits for the cold vapor elements without compromising performance of non-hydride forming elements. Finally, it can be used as a conventional cyclonic spray chamber with pneumatic nebulization.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Contact me about the HydroMist

 

 

SeaSpray Nebulizer for ICP

The SeaSpray Nebulizer of Glass Expansion is the best choice when samples contain high concentrations of dissolved solids. Seawater, brine and platinum baths are just a few examples of SeaSpray applications. However, it is not recommended if samples contain large particles or HF. The SeaSpray nebulizer produces the finest aerosol particles, increasing sensitivity.

SeaSpray Nebulizer – high performance nebulizer for ICP-OES and ICP-MS

Advantages of the SeaSpray nebulizer:

  • The highest sensitivity for each concentric nebulizer on the market
  • Excellent analytical performance
  • Excellent atomization efficiency
  • Perfect for high concentrations of dissolved solids
  • High tolerance for particles
  • The fastest possible rinsing because the sample channel is uniform from the entry point to the point.
  • Quick and easy connection to an LC, IC or HPLC without dead volume when combined with the Glass Expansion LC fitting kit.

Because the SeaSpray is equipped with an accurate VitriCone sample capillary:

  • Is it vibration resistant for the best analytical precision?
  • It has an even internal diameter and a blockage-resistant sample channel
  • Let that consistent Nebulizer-to-Nebulizer
  • Performance see through the highest dimensional tolerances

The SeaSpray Direct Connection (DC) nebulizer is the latest design. It has a UniFit sample connector that glides easily over the sample arm and an argon connector that is configured to connect directly to your ICP.

Advantages of the SeaSpray DC Nebulizer are:

  • Inert metal-free argon connector
  • Instrument-specific Direct Connect flexible argon line
  • Reliable ratchet attachment for leakproof gas connection

DC versions of SeaSpray, MicroMist, Conikal, Slurry, DuraMist, OpalMist and VeeSpray Nebulizers are available for the most common models of ICP-OES and ICP-MS.

New module Chronect Workstation Mosh-Moah – AlOx Clean-Up

Automated removal of natural alkanes

Schematic representation of AlOx Trap in Mosh-Moah analysis

Foods contain natural alkanes which, if not recognized, are included in the determination of MOSH. Axel Semrau has developed an automated solution for this, which can be integrated as an additional module in the Chronect Workstation Mosh-Moah.

The cleaning is done with an additional purification column containing alumina column and removing natural hydrocarbons. This solution requires an additional Valve and HPLC pump for the Mosh-Moah. The cleaning can be combined with the automatic epoxidation step. This step prevents natural olefins from entering the MOAH fraction.

 

 

Analysis of sunflower oil; display of peaks before and after purification with aluminium oxide

 

 

Shelf life standards up to four years

Standards of Inorganic Ventures have always had a certified shelf life of one year. With new technology it is now possible to store stock standards for ICP-OES, ICP-MS and AAS for up to 4 years without changing the concentration. These 4 years start when a batch is produced. It is therefore not the case that these can be kept for another 4 years when you receive the standard. However, they strive to deliver stock standards that have a shelf life of at least two years when they are delivered to the customer. However, this cannot be guaranteed. Of course, we still guarantee a shelf life of at least one year.

Background; what is transpiration?

Transpiration refers to the wall of a bottle that allows water vapour to pass through and/or through evaporation from the closed bottle opening. It leads to an increase in the concentration of the CRM/RM.

The solution

Transpiration Control Technology extends the shelf life of the product. Inorganic Ventures uses a special aluminized bag that prevents an increase in concentration until the bag is opened. The sealed TCT bag stops the loss of water vapour from the bottle when equilibrium is reached in the bag. So it’s the same high Inorganic Ventures TCT zakkenquality product as you’ve come to expect from them, but even more improved.

TCT is an investment by Inorganic Ventures that extends the shelf life of the stock standards and gives you more control over your standards without it costing you extra. The unconditional guarantee of the products remains the same.

What does this mean for you?

Stock standards that you receive from now on will be packed in a TCT bag. In the sealed bag, the stock standard is certified for four years. When you open the bag, the standard is certified for one year. After receiving the product, you keep the bag closed until you start using it. The bag states how long you can use the product before it expires.

You can find the full explanation on the Inorganic Ventures website.