UV-Vis Spectrophotometers

Cole Parmer offers five series of visible and UV/Vis spectrophotometers ranging from entry-level Single Beam, Split-Beam with a spectral bandwidth of 1.5nm and a more advanced variable Double Beam spectrophotometer with a spectral bandwidth of up to 0.5nm. Previously, these were known under the Jenway brand.

UV and Visible spectrophotometry is one of the most widely used techniques in analytical science. Procedures using this method are important for analytical, clinical, and research laboratories, and have intensive application in a Quality Assurance environment. The technique is fundamentally accurate and precise, but it is essential to regularly check the performance of the instrument to see if it is still within the indicated specifications. If necessary, action can then be taken if it appears that the measurement results are out of bounds.

Each instrument is tested by a product specialist before installation to ensure that it can be used immediately in your lab.

In the overview below you can find the right model based on the desired characteristics.

1. SP-200 Series Spectrophotometers

The SP-200 series offers a comprehensive selection of spectrophotometers, with each model covering a specific spectrum range. First, there is model SP-200-VIS, which focuses on the visible spectrum, allowing detailed analysis within this range. Next, is model SP-250-VIS, which is equipped with a dome-shaped lid for enhanced performance. For those wishing to explore the UV region, there is model SP-200-UV, which uses a pulsed xenon lamp to extend the wavelength range.

Remarkably, all models feature measurement modes for absorbance, percent transmission and concentration, highlighting their versatility. This makes these spectrophotometers particularly suitable for a variety of applications. For educational purposes, such as in schools and universities, they provide a solid foundation for photometric analysis. Moreover, they are equally invaluable in quality control laboratories, where precision and reliability are of paramount importance.

Key features

The domed lid of the SP-250-VIS is suitable for tubes up to 105 mm
SP-200-UV uses a high-quality pulsed xenon lamp
Simple and intuitive operation

2. SP-300 Series spectrophotometers; comprehensive options for precise analysis

The SP-300 series of spectrophotometers consists of four models:

  1. SP-300-VIS: Coverage of the visible spectrum.
  2. SP-350-VIS: Extensive options for visible spectrum coverage.
  3. SP-300-UV: Deployment of flash xenon lamp for UV coverage.
  4. SP-350-UV: UV range with advanced features.

Basic Models and Advanced Options:

  • SP-300-VIS and SP-300-UV: Basic models with measurement modes for percent transmission, absorption and concentration.
  • SP-350-VIS and SP-350-UV: Advanced models with basic functions and added capabilities such as scanning, kinetics and quantification.

Optical enhancements for accurate results:

  • All SP-300 models feature enhanced optics.
  • Spectral bandwidth of only 5 nm for precision.
  • Wide absorption range of -0.3 to 2.5 Abs for detailed analysis.

Discover the versatility and accuracy of the SP-300 series spectrophotometers for various analytical applications.


  • Models SP-300-UV and SP-350-UV use a press-to-read xenon lamp for more accurate readings and longer lamp life.
  • The xenon lamp has a 3-year warranty.
  • SP-300-VIS and SP-350-VIS models have a tungsten halogen lamp with a lamp saver feature that automatically turns the lamp off after periods of inactivity.

3. SP-400 spectrophotometers.

The SP-400 series spectrophotometers are the first scanning spectrophotometers in the Cole-Parmer range that use diode array technology to produce exceptionally fast results. The series includes two models. Model SP-400-VIS covers a wavelength range of 335 to 800 nm with a spectral bandwidth of 7 nm. Model SP-400-UV covers a wavelength range of 198 to 800 nm with a spectral bandwidth of 5 nm.

Key features.

  • Scanning diode array technology
  • Navigation with color touch screen
  • Small footprint and lightweight
  • Fast scanning speed
  • English, French and German language options
  • Multiple USB ports for data storage and printer connection
  • Wide range of accessories available

4. SP-500 and SP-600 spectrophotometers.

The SP-500 and SP-600 series spectrophotometers deliver precise and reliable results in a variety of application areas. They are suitable for education, industrial applications and routine sample analysis in quality control environments. In addition, these spectrophotometers are designed with ease of use in mind and offer enhanced connectivity for effortless and fast data transfer.

The series includes a range of models. The SP-500-VIS model uses a tungsten halogen lamp and makes measurements in the visible spectrum, ranging from 320 to 1000 nm. In contrast, models SP-500-UV, SP-500-NANO and SP-600-UV use a xenon lamp to make measurements in the UV spectrum, up to 198 nm.

Within the SP-600 series, the spectrophotometers are equipped with split-beam optics, also known as ratio or double-beam. This innovation provides improved accuracy and reproducibility. The split beam acts as a correction mechanism for fluctuations, resulting in stabilized measurement reliability over extended periods of time. This makes these spectrophotometers excellent for time lapse and kinetic measurements.

Key features:

  • 7-inch high-definition color touchscreen display
  • Multiple USB ports for data storage and printer connectivity
  • Android operating system
  • Internal memory for methods and results (10 GB on-board storage)
  • Multiple language options including English, French, German, Spanish and Italian
  • Extensive range of accessories available
  • Pre-programmed for DNA, RNA and protein analysis (SP-500-NANO only)
  • Only a few μL of sample required for measurement (SP-500-NANO only)
Cole Parmer

5. SP-800 UV-Visible Double-Beam Spectrophotometer.

The SP-800-UV is a UV-Visible double-beam spectrophotometer equipped with a variable spectral bandwidth. In addition, this spectrophotometer features an integrated user interface, enabling local operation and ease of navigation. Thanks to the highly stable optics and the presence of two detectors, both the sample and the reference can be measured simultaneously. This optimizes measurement accuracy.

The SP-800-UV offers various measurement modes, such as photometry, concentration, multiwavelength, spectrum scanning, kinetics, quantitation, DNA and protein analysis. In the multi-wavelength measurement mode, even up to 10 wavelengths can be measured, while quantitation curves can be created by measuring up to 10 standards at 3 different wavelengths.

This dual-beam spectrophotometer is ideally suited for a variety of applications including quality control, general research, pharmaceutical, biochemical and clinical laboratory applications.

Key Features:

  • Double-beam spectrophotometer with highly stable optics
  • Integrated user interface for local operation
  • Variable spectral bandwidth 0.5, 1, 2, 4 and 5 nm
  • Prism PC software included as standard
  • Wide range of accessories available
  • 1 year warranty

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