{"id":47614,"date":"2025-10-22T11:32:04","date_gmt":"2025-10-22T09:32:04","guid":{"rendered":"https:\/\/www.instrument-solutions.com\/technology\/chromatography\/gpc-sec-chromatography"},"modified":"2025-10-22T11:32:24","modified_gmt":"2025-10-22T09:32:24","slug":"gpc-sec-chromatography","status":"publish","type":"page","link":"https:\/\/www.instrument-solutions.com\/en\/technology\/chromatography\/gpc-sec-chromatography","title":{"rendered":"GPC-SEC chromatography"},"content":{"rendered":"<div  class='flex_column av-h8hdnhg-d7fbb44cc00793435c81f82cd0b87418 av_one_full  avia-builder-el-0  el_before_av_toggle_container  avia-builder-el-first  first flex_column_div  '     ><p>\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-llqpdjzu-c2468aa89ec55bfa9e36a9f01ae60eb8\">\n#top .av-special-heading.av-llqpdjzu-c2468aa89ec55bfa9e36a9f01ae60eb8{\npadding-bottom:10px;\n}\nbody .av-special-heading.av-llqpdjzu-c2468aa89ec55bfa9e36a9f01ae60eb8 .av-special-heading-tag .heading-char{\nfont-size:25px;\n}\n.av-special-heading.av-llqpdjzu-c2468aa89ec55bfa9e36a9f01ae60eb8 .av-subheading{\nfont-size:15px;\n}\n<\/style>\n<div  class='av-special-heading av-llqpdjzu-c2468aa89ec55bfa9e36a9f01ae60eb8 av-special-heading-h2  avia-builder-el-1  el_before_av_textblock  avia-builder-el-first '><h2 class='av-special-heading-tag '  itemprop=\"headline\"  >AZURA\u00ae GPC <span class='special_amp'>&amp;<\/span> SEC chromatography<\/h2><div class=\"special-heading-border\"><div class=\"special-heading-inner-border\"><\/div><\/div><\/div><br \/>\n<section  class='av_textblock_section av-gmhak9w-21e95c976f177109829fa043f7dc819d '   itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/CreativeWork\" ><div class='avia_textblock'  itemprop=\"text\" ><h4>Gel permeation chromatography (GPC) &amp; and Size Exclusion chromatography (SE<\/h4>\n<h4><img decoding=\"async\" class=\"alignleft\" src=\"https:\/\/www.instrument-solutions.com\/wp-content\/uploads\/Knauer-logo-250px.png\" alt=\"\" width=\"250\" height=\"40\" \/><\/h4>\n<p>The KNAUER line of GPC-SEC chromatography systems, called AZURA\u00ae SEC, provides solutions for a variety of applications and laboratories. These systems perform analysis, separate components from samples and fractionate them by size and molecular weight. This makes them valuable in industries as diverse as polymer production, food, biochemistry and pharmaceuticals, and equally valuable in research settings.<\/p>\n<p>These systems have several variants, each tailored to specific laboratory needs. For example, the AZURA SEC Compact is designed for cost-conscious solutions, while the AZURA SEC Lab provides optimal analytical performance.<\/p>\n<blockquote>\n<p>Robust system solutions for your GPC\/SEC analysis, fractionation and GPC cleanup needs<\/p>\n<\/blockquote>\n<h4>Features<\/h4>\n<ul>\n<li>Optimized systems for GPC\/SEC applications<\/li>\n<li>Suitable for organic and aqueous solventsHighly accurate double-piston pump with extra-low pulsation<\/li>\n<li>Sensitive refractive index detector optimized for low drift<\/li>\n<li>Dedicated GPC\/SEC software based on ClarityChrom<\/li>\n<li>Wide range of detector types available<\/li>\n<li>Optionally available degasser suitable for organic solvents<\/li>\n<\/ul>\n<\/div><\/section><br \/>\n<div  class='avia-button-wrap av-giahgqs-5b5b6e18f6d0666ec8d3f54c59ef184f-wrap avia-button-left  avia-builder-el-3  el_after_av_textblock  el_before_av_hr '><a href='\/wp-content\/uploads\/Knauer-AZURA-SEC-brochure.pdf'  class='avia-button av-giahgqs-5b5b6e18f6d0666ec8d3f54c59ef184f av-link-btn avia-icon_select-yes-left-icon avia-size-medium avia-position-left avia-color-theme-color'   aria-label=\"brochure KNAUER AZURA GPC, SEC System Solutions\"><span class='avia_button_icon avia_button_icon_left avia-iconfont avia-font-entypo-fontello' data-av_icon='\ue82d' data-av_iconfont='entypo-fontello' ><\/span><span class='avia_iconbox_title' >brochure KNAUER AZURA GPC, SEC System Solutions<\/span><\/a><\/div><br \/>\n<div  class='hr av-fp8tuzo-e69027732c141c5fb742896e5e13dc9d hr-default  avia-builder-el-4  el_after_av_button  avia-builder-el-last '><span class='hr-inner '><span class=\"hr-inner-style\"><\/span><\/span><\/div><\/p><\/div>\n\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-fe7aqdg-047212ccd930bbe7c36317098630b9b8\">\n#top .togglecontainer.av-fe7aqdg-047212ccd930bbe7c36317098630b9b8 p.toggler{\nbackground-color:#eded6f;\nborder-color:#3a8ee8;\n}\n#top .togglecontainer.av-fe7aqdg-047212ccd930bbe7c36317098630b9b8 .toggle_wrap .toggle_content{\nbackground-color:#eded6f;\nborder-color:#3a8ee8;\n}\n<\/style>\n<div  class='togglecontainer av-fe7aqdg-047212ccd930bbe7c36317098630b9b8  avia-builder-el-5  el_after_av_one_full  el_before_av_one_full  toggle_close_all'  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/FAQPage\" >\n<section class='av_toggle_section av-f3fetr8-48d84560eadfaffe395e68f46909a2ab'  itemscope=\"itemscope\" itemprop=\"mainEntity\" itemtype=\"https:\/\/schema.org\/Question\" ><div role=\"tablist\" class=\"single_toggle\" data-tags=\"{All} \"  ><p id='toggle-toggle-id-1' data-fake-id='#toggle-id-1' class='toggler  av-title-above av-inherit-border-color'  itemprop=\"name\"  role='tab' tabindex='0' aria-controls='toggle-id-1' data-slide-speed=\"200\" data-title=\" Principle of GPC\/SEC\" data-title-open=\"\" data-aria_collapsed=\"Click to expand:  Principle of GPC\/SEC\" data-aria_expanded=\"Click to collapse:  Principle of GPC\/SEC\"> Principle of GPC\/SEC<span class=\"toggle_icon\"><span class=\"vert_icon\"><\/span><span class=\"hor_icon\"><\/span><\/span><\/p><div id='toggle-id-1' aria-labelledby='toggle-toggle-id-1' role='region' class='toggle_wrap  av-title-above'   itemscope=\"itemscope\" itemprop=\"acceptedAnswer\" itemtype=\"https:\/\/schema.org\/Answer\" ><div class='toggle_content invers-color av-inherit-border-color'  itemprop=\"text\" ><h4>How does GPC\/SEC work?<\/h4>\n<p>Gel permeation chromatography (GPC) and size-exclusion chromatography (SEC) <strong>separate analytes by size<\/strong> using special columns with a porous matrix. The basic principle of separation is based on the fact that smaller molecules are more likely to penetrate the pores, so their travel time through the matrix is longer than that of larger molecules. Separations (usually of a mixture of polymers, polysaccharides or biomolecules) are facilitated by choosing the optimal particle and pore size and column length. GPC\/SEC is often used for <strong>quality control<\/strong> and <strong>R&amp;D<\/strong>.<\/p>\n<p>The solvents used in polymer analysis are chosen based on their ability to dissolve polymeric substances &#8211; and therefore may damage certain components of a device. And the use of salt buffers in SEC is damaging to metal parts of the system. Therefore, all parts of the system that come into contact with liquid, from valves and seals to hoses, must be carefully considered when developing such systems.<\/p>\n<\/div><\/div><\/div><\/section>\n<section class='av_toggle_section av-ebr8cdg-15df531e02aafbb92aa2e10a83fec6f9'  itemscope=\"itemscope\" itemprop=\"mainEntity\" itemtype=\"https:\/\/schema.org\/Question\" ><div role=\"tablist\" class=\"single_toggle\" data-tags=\"{All} \"  ><p id='toggle-toggle-id-2' data-fake-id='#toggle-id-2' class='toggler  av-title-above av-inherit-border-color'  itemprop=\"name\"  role='tab' tabindex='0' aria-controls='toggle-id-2' data-slide-speed=\"200\" data-title=\"What do I need for GPC?\" data-title-open=\"\" data-aria_collapsed=\"Click to expand: What do I need for GPC?\" data-aria_expanded=\"Click to collapse: What do I need for GPC?\">What do I need for GPC?<span class=\"toggle_icon\"><span class=\"vert_icon\"><\/span><span class=\"hor_icon\"><\/span><\/span><\/p><div id='toggle-id-2' aria-labelledby='toggle-toggle-id-2' role='region' class='toggle_wrap  av-title-above'   itemscope=\"itemscope\" itemprop=\"acceptedAnswer\" itemtype=\"https:\/\/schema.org\/Answer\" ><div class='toggle_content invers-color av-inherit-border-color'  itemprop=\"text\" ><p>GPC columns are usually long and runs are performed at low flow rates up to 1 ml\/min and at low (&lt;100 bar) pressures. For accurate molecular weight determination, which is the goal of most GPC\/SEC users, a <strong>good isocratic pump<\/strong> is essential for a <strong>stable baseline<\/strong>. Also, a <strong>degasser<\/strong> suitable for a wide range of partially highly aggressive solvents is very important for reproducible results. In addition, temperature is critical for many GPC applications, so a thermostat to control column temperature is essential.<\/p>\n<p>Because very aggressive media are often used in polymer analysis, all wetted components must be compatible with the solvent used. Depending on the polymer, it is important to consider this when configuring a GPC system.<\/p>\n<\/div><\/div><\/div><\/section>\n<section class='av_toggle_section av-dv70v6s-eff63358ae4c58c4d66d15a78934400e'  itemscope=\"itemscope\" itemprop=\"mainEntity\" itemtype=\"https:\/\/schema.org\/Question\" ><div role=\"tablist\" class=\"single_toggle\" data-tags=\"{All} \"  ><p id='toggle-toggle-id-3' data-fake-id='#toggle-id-3' class='toggler  av-title-above av-inherit-border-color'  itemprop=\"name\"  role='tab' tabindex='0' aria-controls='toggle-id-3' data-slide-speed=\"200\" data-title=\" What is GPC Cleanup?\" data-title-open=\"\" data-aria_collapsed=\"Click to expand:  What is GPC Cleanup?\" data-aria_expanded=\"Click to collapse:  What is GPC Cleanup?\"> What is GPC Cleanup?<span class=\"toggle_icon\"><span class=\"vert_icon\"><\/span><span class=\"hor_icon\"><\/span><\/span><\/p><div id='toggle-id-3' aria-labelledby='toggle-toggle-id-3' role='region' class='toggle_wrap  av-title-above'   itemscope=\"itemscope\" itemprop=\"acceptedAnswer\" itemtype=\"https:\/\/schema.org\/Answer\" ><div class='toggle_content invers-color av-inherit-border-color'  itemprop=\"text\" ><p>GPC Cleanup is a sample preparation method based on gel permeation chromatography (GPC). It is mainly used to perform<strong> general cleaning tasks<\/strong> on a wide range of sample matrices such as food, tissue, plant and environmental samples. <strong>High-weight molecular substances<\/strong> such as lipids, proteins, natural resins, cellular components and steroids, which interfere with subsequent analysis of pesticides, for example, are <strong>efficiently removed<\/strong>.<\/p>\n<\/div><\/div><\/div><\/section>\n<\/div>\n<div  class='flex_column av-dhmr9z8-0e9d338d1176661cfc2f551c9f00baf5 av_one_full  avia-builder-el-6  el_after_av_toggle_container  el_before_av_hr  first flex_column_div  '     ><style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-d8f6z6s-1b83792baba9be6909366e7d55c3c3d6\">\n.avia-image-container.av-d8f6z6s-1b83792baba9be6909366e7d55c3c3d6 img.avia_image{\nbox-shadow:none;\n}\n.avia-image-container.av-d8f6z6s-1b83792baba9be6909366e7d55c3c3d6 .av-image-caption-overlay-center{\ncolor:#ffffff;\n}\n<\/style>\n<div  class='avia-image-container av-d8f6z6s-1b83792baba9be6909366e7d55c3c3d6 av-styling- avia-align-center  avia-builder-el-7  avia-builder-el-no-sibling '   itemprop=\"image\" itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/ImageObject\" ><div class=\"avia-image-container-inner\"><div class=\"avia-image-overlay-wrap\"><img fetchpriority=\"high\" decoding=\"async\" fetchpriority=\"high\" fetchpriority=\"high\" class='wp-image-19497 avia-img-lazy-loading-not-19497 avia_image ' src=\"https:\/\/www.instrument-solutions.com\/wp-content\/uploads\/Knauer-GPC-systeempresentatie.jpg\" alt='presentatie van een Knauer Azura GPC\/SEC opstelling' title='Knauer GPC systeempresentatie'  height=\"849\" width=\"995\"  itemprop=\"thumbnailUrl\" srcset=\"https:\/\/www.instrument-solutions.com\/wp-content\/uploads\/Knauer-GPC-systeempresentatie.jpg 995w, https:\/\/www.instrument-solutions.com\/wp-content\/uploads\/Knauer-GPC-systeempresentatie-300x256.jpg 300w, https:\/\/www.instrument-solutions.com\/wp-content\/uploads\/Knauer-GPC-systeempresentatie-768x655.jpg 768w, https:\/\/www.instrument-solutions.com\/wp-content\/uploads\/Knauer-GPC-systeempresentatie-705x602.jpg 705w\" sizes=\"(max-width: 995px) 100vw, 995px\" \/><\/div><\/div><\/div><\/div>\n<div  class='hr av-cobzqo4-8d6a6e6473f3ade20f70dfde930aa8a3 hr-default  avia-builder-el-8  el_after_av_one_full  el_before_av_one_full '><span class='hr-inner '><span class=\"hr-inner-style\"><\/span><\/span><\/div>\n<div  class='flex_column av-cdsdrd0-ab62b6a014818d998476375bba927a13 av_one_full  avia-builder-el-9  el_after_av_hr  el_before_av_one_half  first flex_column_div  '     ><section  class='av_textblock_section av-bvf5qx0-c82077867fd95406a53fa77b49cd2d15 '   itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/CreativeWork\" ><div class='avia_textblock'  itemprop=\"text\" ><h4>4 examples of AZURA GPC\/SEC Systems:<\/h4>\n<\/div><\/section><\/div>\n<div  class='flex_column av-becsjs4-09573ab6bee494d91d1ef4fad97dda9d av_one_half  avia-builder-el-11  el_after_av_one_full  el_before_av_one_half  first flex_column_div  column-top-margin'     ><section  class='av_textblock_section av-aw9r1s4-0ae02d5f7a9036de49e01e2fcd733d2e '   itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/CreativeWork\" ><div class='avia_textblock'  itemprop=\"text\" ><h4>AZURA SEC Compact Systems &#8211; simple and attractively priced with full analytical GPC potential<\/h4>\n<p>AZURA\u00ae SEC Compact systems present a remarkable balance between price and performance, making them the optimal choice for any laboratory entering the world of GPC\/SEC analysis. These systems find application in process control, as well as in laboratories with limited financial resources and educational laboratories.<\/p>\n<p>The core component of the systems is the Assistant ASM 2.2L, a docking station that accommodates up to three compact devices. Within one enclosure, valves, pumps and UV detectors can be combined. Users can easily perform routine maintenance, such as replacing a lamp in a detector. The modular design allows additional components to be added to the systems at any time. Here, the docking station comes with a pump including a pressure sensor and a valve actuator with injection valve for accurate sample feeding.<\/p>\n<h4>Key features<\/h4>\n<ul>\n<li>Flexible, easily expandable modular concept<\/li>\n<li>Suitable for organic and aqueous eluents<\/li>\n<li>Injection via valve actuator for minimal pressure spikes<\/li>\n<li>Powerful RI detector<\/li>\n<li>Column thermostat CT 2.1<\/li>\n<\/ul>\n<h4>Upgrade options<\/h4>\n<ul>\n<li>Save on operating costs with automatic solvent recycling valve<\/li>\n<li>Degasser<\/li>\n<li>Automatic samplerDetectors (RI, UV, ELSD, PDA, FLD, ECD)<\/li>\n<\/ul>\n<\/div><\/section><\/div>\n<div  class='flex_column av-af33j38-b1458b6477b9fc5434117a16ba03dfd4 av_one_half  avia-builder-el-13  el_after_av_one_half  el_before_av_hr  flex_column_div  column-top-margin'     ><style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-9tfr484-55e9815a64f290deb8d5a6f172f1acb5\">\n.avia-image-container.av-9tfr484-55e9815a64f290deb8d5a6f172f1acb5 img.avia_image{\nbox-shadow:none;\n}\n.avia-image-container.av-9tfr484-55e9815a64f290deb8d5a6f172f1acb5 .av-image-caption-overlay-center{\ncolor:#ffffff;\n}\n<\/style>\n<div  class='avia-image-container av-9tfr484-55e9815a64f290deb8d5a6f172f1acb5 av-styling- avia_animated_image av-animated-when-visible-95 fade-in avia-align-center  avia-builder-el-14  avia-builder-el-no-sibling '   itemprop=\"image\" itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/ImageObject\" ><div class=\"avia-image-container-inner\"><div class=\"avia-image-overlay-wrap\"><img fetchpriority=\"high\" decoding=\"async\" fetchpriority=\"high\" fetchpriority=\"high\" class='wp-image-19506 avia-img-lazy-loading-not-19506 avia_image ' src=\"https:\/\/www.instrument-solutions.com\/wp-content\/uploads\/Knauer-AZURA-SEC-Compact-P4.1S.jpg\" alt='' title='Knauer AZURA SEC Compact P4.1S'  height=\"300\" width=\"300\"  itemprop=\"thumbnailUrl\" srcset=\"https:\/\/www.instrument-solutions.com\/wp-content\/uploads\/Knauer-AZURA-SEC-Compact-P4.1S.jpg 300w, https:\/\/www.instrument-solutions.com\/wp-content\/uploads\/Knauer-AZURA-SEC-Compact-P4.1S-150x150.jpg 150w, https:\/\/www.instrument-solutions.com\/wp-content\/uploads\/Knauer-AZURA-SEC-Compact-P4.1S-36x36.jpg 36w, https:\/\/www.instrument-solutions.com\/wp-content\/uploads\/Knauer-AZURA-SEC-Compact-P4.1S-180x180.jpg 180w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/div><\/div><\/div><\/div>\n<div  class='hr av-99q2f50-89687ad41acb1b355cfde4abd237b61a hr-default  avia-builder-el-15  el_after_av_one_half  el_before_av_one_half '><span class='hr-inner '><span class=\"hr-inner-style\"><\/span><\/span><\/div>\n<div  class='flex_column av-8v3zt0k-13c3c73766db877b81c3cb2c4f495b5c av_one_half  avia-builder-el-16  el_after_av_hr  el_before_av_one_half  first flex_column_div  '     ><section  class='av_textblock_section av-8h52ybo-20bf50098dfe266ee1cefeb0a0dbaa68 '   itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/CreativeWork\" ><div class='avia_textblock'  itemprop=\"text\" ><h4>AZURA SEC Lab &#8211; reliable and flexible for even the most demanding GPC separations<\/h4>\n<p>AZURA\u00ae SEC Lab systems are preferred by laboratories demanding extremely high analytical performance. Incredible flow precision is combined with ultra-low pulsation in the system, resulting in impressive precision and reproducibility of retention volume.<\/p>\n<p>Laboratories requiring extremely high analytical performance prefer to choose the AZURA\u00ae SEC Lab systems. The system combines incredible flow precision with ultra-low pulsation, and this interplay results in impressive precision and reproducibility of retention volume.<\/p>\n<h4>Large capacity<\/h4>\n<p>With the autosampler, it is possible to inject up to 768 positions via microtiter plates, or use 108 standard 1.5-mL sample vials. This makes the autosampler exceptionally suitable for larger sample volumes, and it is the ideal option for laboratories with a large sample throughput. Its reliable and robust design ensures that it acts as a tireless force in any laboratory.<\/p>\n<h4>Key features<\/h4>\n<ul>\n<li>Unsurpassed flow precision combined with ultra-low pulsation for<br \/>\nHighest analytical performance<\/li>\n<li>Suitable for organic and aqueous eluents<\/li>\n<li>Highest level of automation<\/li>\n<li>Expandable modular concept with a wide range of detectors<\/li>\n<\/ul>\n<p><strong>Upgrade options<\/strong><\/p>\n<ul>\n<li>Save on operational costs with automatic solvent recycling valve<\/li>\n<li>Degasser<\/li>\n<li>Detector (RI, UV, ELSD, PDA, FLD, ECD)<\/li>\n<\/ul>\n<\/div><\/section><\/div>\n<div  class='flex_column av-7v6fays-f52be678ca836fcfab68306fd0ab9382 av_one_half  avia-builder-el-18  el_after_av_one_half  el_before_av_hr  flex_column_div  '     ><style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-7n0q690-48789302b67160b55b1cd03813974882\">\n.avia-image-container.av-7n0q690-48789302b67160b55b1cd03813974882 img.avia_image{\nbox-shadow:none;\n}\n.avia-image-container.av-7n0q690-48789302b67160b55b1cd03813974882 .av-image-caption-overlay-center{\ncolor:#ffffff;\n}\n<\/style>\n<div  class='avia-image-container av-7n0q690-48789302b67160b55b1cd03813974882 av-styling- avia_animated_image av-animated-when-visible-95 fade-in avia-align-center  avia-builder-el-19  avia-builder-el-no-sibling '   itemprop=\"image\" itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/ImageObject\" ><div class=\"avia-image-container-inner\"><div class=\"avia-image-overlay-wrap\"><img fetchpriority=\"high\" decoding=\"async\" fetchpriority=\"high\" fetchpriority=\"high\" class='wp-image-19525 avia-img-lazy-loading-not-19525 avia_image ' src=\"https:\/\/www.instrument-solutions.com\/wp-content\/uploads\/Knauer-AZURA-SEC-LAB-System.jpg\" alt='' title='Knauer AZURA SEC LAB System'  height=\"300\" width=\"158\"  itemprop=\"thumbnailUrl\"  \/><\/div><\/div><\/div><\/div>\n<div  class='hr av-71csbs4-f9341da2f2b8fe25e9d3285347255376 hr-default  avia-builder-el-20  el_after_av_one_half  el_before_av_one_half '><span class='hr-inner '><span class=\"hr-inner-style\"><\/span><\/span><\/div>\n<div  class='flex_column av-6p5p550-0f193b5702593eaf7072468ff7c2fe19 av_one_half  avia-builder-el-21  el_after_av_hr  el_before_av_one_half  first flex_column_div  '     ><section  class='av_textblock_section av-63d445g-32aff78ef708df7d8bf68e68b17356a1 '   itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/CreativeWork\" ><div class='avia_textblock'  itemprop=\"text\" ><h4>AZURA SEC Semi-Prep &#8211; automatic separation and fraction collection<\/h4>\n<div class=\"flex-1 overflow-hidden\">\n<div class=\"react-scroll-to-bottom--css-nwdxk-79elbk h-full dark:bg-gray-800\">\n<div class=\"react-scroll-to-bottom--css-nwdxk-1n7m0yu\">\n<div class=\"flex flex-col text-sm dark:bg-gray-800\">\n<div class=\"group w-full text-token-text-primary border-b border-black\/10 dark:border-gray-900\/50 bg-gray-50 dark:bg-&#091;#444654&#093;\">\n<div class=\"flex p-4 gap-4 text-base md:gap-6 md:max-w-2xl lg:max-w-&#091;38rem&#093; xl:max-w-3xl md:py-6 lg:px-0 m-auto\">\n<div class=\"relative flex w-&#091;calc(100%-50px)&#093; flex-col gap-1 md:gap-3 lg:w-&#091;calc(100%-115px)&#093;\">\n<div class=\"flex flex-grow flex-col gap-3 max-w-full\">\n<div class=\"min-h-&#091;20px&#093; flex flex-col items-start gap-3 overflow-x-auto whitespace-pre-wrap break-words\">\n<div class=\"markdown prose w-full break-words dark:prose-invert light\">\n<p>AZURA\u00ae SEC Semi-prep systems offer the ability to fractionate specific molecular weight ranges or perform other purification tasks. These systems can deliver flow rates up to 50 ml\/min thanks to a pump, making them suitable for virtually any semi-prep GPC\/SEC column.<\/p>\n<p>The modular design of the AZURA platform and the special PurityChrom\u00ae software allow users to flexibly adapt the systems to various separation tasks.<\/p>\n<p>Fraction collection can be performed in several ways:<\/p>\n<ul>\n<li>Manual: Direct control for fraction collection<\/li>\n<li>Volume-based: Collection at set volumes<\/li>\n<li>Peak-based: Collection based on detector signal<\/li>\n<li>From switching valve to fraction collector solutions<\/li>\n<\/ul>\n<h4>Key features include:<\/h4>\n<ul>\n<li>Modular concept suitable for various separation and purification tasks<\/li>\n<li>Flow rates up to 50 ml\/min<\/li>\n<li>Automatic sampler with up to 10 ml injection volume<\/li>\n<li>Suitability for both organic and aqueous eluents<\/li>\n<li>Choice between manual sample injection or automatic sampler<\/li>\n<li>Option for single or multiple fractions<\/li>\n<li>Cost savings with optional automatic valve for solvent recycling<\/li>\n<\/ul>\n<h4>Available detectors for Semi-prep include:<\/h4>\n<ul>\n<li>Special high-flow RI detector (as in this system proposal), usable up to 100 ml\/min<\/li>\n<li>UV detectors UVD 2.1S and UVD 2.1L<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div><\/section><\/div>\n<div  class='flex_column av-5mdq31g-29d264008cc943e466b250cc8dd6a37e av_one_half  avia-builder-el-23  el_after_av_one_half  el_before_av_hr  flex_column_div  '     ><style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-559oves-d05365ad4c2464f9041c703aa27c4aa6\">\n.avia-image-container.av-559oves-d05365ad4c2464f9041c703aa27c4aa6 img.avia_image{\nbox-shadow:none;\n}\n.avia-image-container.av-559oves-d05365ad4c2464f9041c703aa27c4aa6 .av-image-caption-overlay-center{\ncolor:#ffffff;\n}\n<\/style>\n<div  class='avia-image-container av-559oves-d05365ad4c2464f9041c703aa27c4aa6 av-styling- avia_animated_image av-animated-when-visible-95 fade-in avia-align-center  avia-builder-el-24  avia-builder-el-no-sibling '   itemprop=\"image\" itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/ImageObject\" ><div class=\"avia-image-container-inner\"><div class=\"avia-image-overlay-wrap\"><img fetchpriority=\"high\" decoding=\"async\" fetchpriority=\"high\" fetchpriority=\"high\" class='wp-image-19529 avia-img-lazy-loading-not-19529 avia_image ' src=\"https:\/\/www.instrument-solutions.com\/wp-content\/uploads\/Knauer-AZURA-SEC-Semi-prep-systeem.jpg\" alt='AZURA SEC Semi-Prep systeem' title='Knauer AZURA SEC Semi-prep systeem'  height=\"300\" width=\"300\"  itemprop=\"thumbnailUrl\" srcset=\"https:\/\/www.instrument-solutions.com\/wp-content\/uploads\/Knauer-AZURA-SEC-Semi-prep-systeem.jpg 300w, https:\/\/www.instrument-solutions.com\/wp-content\/uploads\/Knauer-AZURA-SEC-Semi-prep-systeem-150x150.jpg 150w, https:\/\/www.instrument-solutions.com\/wp-content\/uploads\/Knauer-AZURA-SEC-Semi-prep-systeem-36x36.jpg 36w, https:\/\/www.instrument-solutions.com\/wp-content\/uploads\/Knauer-AZURA-SEC-Semi-prep-systeem-180x180.jpg 180w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/div><\/div><\/div><\/div>\n<div  class='hr av-h276qs-d520cb9b5d39a1f2c16771372d6d4bde hr-default  avia-builder-el-25  el_after_av_one_half  el_before_av_one_half '><span class='hr-inner '><span class=\"hr-inner-style\"><\/span><\/span><\/div>\n<div  class='flex_column av-46siilg-155732c3f0d89a20e5f62e713b6a4f96 av_one_half  avia-builder-el-26  el_after_av_hr  el_before_av_one_half  first flex_column_div  '     ><section  class='av_textblock_section av-3v5wn5w-7ecdb8b3e2e3a38318bcd10b7bde96d2 '   itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/CreativeWork\" ><div class='avia_textblock'  itemprop=\"text\" ><h3>AZURA GPC Cleanup \u2013 save time with automatic sample preparation<\/h3>\n<p>Save time and labour by moving away from manual sample preparation. The AZURA\u00ae GPC Cleanup system automates labour-intensive and time-consuming cleaning tasks based on gel permeation chromatography (GPC). It also <strong>improves<\/strong> the <strong>reproducibility<\/strong> and <strong>quality<\/strong> of the <strong>clean-up<\/strong>. After loading the sample, the specialised system performs fully automated processing for up to 15 samples, all while taking up minimal workspace. The system is based on the versatile AZURA\u00ae instrument platform and can be adapted to individual requirements. For intuitive device operation directly on the system, there is the cost-effective Mobile Control software, optimised for touch control.<\/p>\n<p>In accordance with recognised methods for the determination of pesticide residues<\/p>\n<ul>\n<li>Method 984.21 &#8211; AOAC International<\/li>\n<li>EPA SW-846 Method 3640A &#8211; US Environmental Protection Agency<\/li>\n<li>AEN 12393 and EN 1528 \u2013 European standard<\/li>\n<li>L 00.00-34 Method according to \u00a764 LFGB (formerly \u00a7 35 LMBG)<\/li>\n<\/ul>\n<p>The AZURA\u00ae GPC Cleanup system was selected by Analytical Scientist for \u2018The Innovators 2017\u2019 product. Click here to read the full article.<\/p>\n<\/div><\/section><\/div>\n<div  class='flex_column av-3es8xms-3fa05bc4e71cc86e0be885215e963bc6 av_one_half  avia-builder-el-28  el_after_av_one_half  el_before_av_hr  flex_column_div  '     ><style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-am6kas-35b8363880fce8eace101a2aa009e52c\">\n.avia-image-container.av-am6kas-35b8363880fce8eace101a2aa009e52c img.avia_image{\nbox-shadow:none;\n}\n.avia-image-container.av-am6kas-35b8363880fce8eace101a2aa009e52c .av-image-caption-overlay-center{\ncolor:#ffffff;\n}\n<\/style>\n<div  class='avia-image-container av-am6kas-35b8363880fce8eace101a2aa009e52c av-styling- avia_animated_image av-animated-when-visible-95 fade-in avia-align-center  avia-builder-el-29  avia-builder-el-no-sibling '   itemprop=\"image\" itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/ImageObject\" ><div class=\"avia-image-container-inner\"><div class=\"avia-image-overlay-wrap\"><img fetchpriority=\"high\" decoding=\"async\" fetchpriority=\"high\" fetchpriority=\"high\" class='wp-image-19539 avia-img-lazy-loading-not-19539 avia_image ' src=\"https:\/\/www.instrument-solutions.com\/wp-content\/uploads\/Knauer-AZURA-GPC-Cleanup.jpg\" alt='' title='Knauer AZURA GPC Cleanup'  height=\"300\" width=\"300\"  itemprop=\"thumbnailUrl\" srcset=\"https:\/\/www.instrument-solutions.com\/wp-content\/uploads\/Knauer-AZURA-GPC-Cleanup.jpg 300w, https:\/\/www.instrument-solutions.com\/wp-content\/uploads\/Knauer-AZURA-GPC-Cleanup-150x150.jpg 150w, https:\/\/www.instrument-solutions.com\/wp-content\/uploads\/Knauer-AZURA-GPC-Cleanup-36x36.jpg 36w, https:\/\/www.instrument-solutions.com\/wp-content\/uploads\/Knauer-AZURA-GPC-Cleanup-180x180.jpg 180w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/div><\/div><\/div><\/div>\n<div  class='hr av-2obj3es-b561d7720f25ddeba4658417b67b4212 hr-default  avia-builder-el-30  el_after_av_one_half  el_before_av_hr '><span class='hr-inner '><span class=\"hr-inner-style\"><\/span><\/span><\/div>\n<div  class='hr av-27eispw-f6d546e02b8847cd87a9b0c5487a0bc6 hr-default  avia-builder-el-31  el_after_av_hr  el_before_av_one_full '><span class='hr-inner '><span class=\"hr-inner-style\"><\/span><\/span><\/div>\n<div  class='flex_column av-1qpqa6c-9a336f9b127fdaf5829de0521363a7ae av_one_full  avia-builder-el-32  el_after_av_hr  avia-builder-el-last  first flex_column_div  '     ><p>\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-1d2yo84-da08bf2955b753f936217d3f97fb5bbe\">\n#top .av-special-heading.av-1d2yo84-da08bf2955b753f936217d3f97fb5bbe{\npadding-bottom:10px;\n}\nbody .av-special-heading.av-1d2yo84-da08bf2955b753f936217d3f97fb5bbe .av-special-heading-tag .heading-char{\nfont-size:25px;\n}\n.av-special-heading.av-1d2yo84-da08bf2955b753f936217d3f97fb5bbe .av-subheading{\nfont-size:15px;\n}\n<\/style>\n<div  class='av-special-heading av-1d2yo84-da08bf2955b753f936217d3f97fb5bbe av-special-heading-h2  avia-builder-el-33  el_before_av_codeblock  avia-builder-el-first '><h2 class='av-special-heading-tag '  itemprop=\"headline\"  >Contact Form<\/h2><div class=\"special-heading-border\"><div class=\"special-heading-inner-border\"><\/div><\/div><\/div><br \/>\n<\/p><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":2,"featured_media":0,"parent":8909,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-47614","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.0 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>GPC-SEC chromatography - Instrument Solutions<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.instrument-solutions.com\/en\/technology\/chromatography\/gpc-sec-chromatography\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"GPC-SEC chromatography - Instrument Solutions\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.instrument-solutions.com\/en\/technology\/chromatography\/gpc-sec-chromatography\" \/>\n<meta property=\"og:site_name\" content=\"Instrument Solutions\" \/>\n<meta property=\"article:modified_time\" content=\"2025-10-22T09:32:24+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.instrument-solutions.com\/wp-content\/uploads\/Knauer-logo-250px.png\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"19 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/www.instrument-solutions.com\\\/en\\\/technology\\\/chromatography\\\/gpc-sec-chromatography\",\"url\":\"https:\\\/\\\/www.instrument-solutions.com\\\/en\\\/technology\\\/chromatography\\\/gpc-sec-chromatography\",\"name\":\"GPC-SEC chromatography - Instrument Solutions\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/www.instrument-solutions.com\\\/en#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\\\/\\\/www.instrument-solutions.com\\\/en\\\/technology\\\/chromatography\\\/gpc-sec-chromatography#primaryimage\"},\"image\":{\"@id\":\"https:\\\/\\\/www.instrument-solutions.com\\\/en\\\/technology\\\/chromatography\\\/gpc-sec-chromatography#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/www.instrument-solutions.com\\\/wp-content\\\/uploads\\\/Knauer-logo-250px.png\",\"datePublished\":\"2025-10-22T09:32:04+00:00\",\"dateModified\":\"2025-10-22T09:32:24+00:00\",\"breadcrumb\":{\"@id\":\"https:\\\/\\\/www.instrument-solutions.com\\\/en\\\/technology\\\/chromatography\\\/gpc-sec-chromatography#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/www.instrument-solutions.com\\\/en\\\/technology\\\/chromatography\\\/gpc-sec-chromatography\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\\\/\\\/www.instrument-solutions.com\\\/en\\\/technology\\\/chromatography\\\/gpc-sec-chromatography#primaryimage\",\"url\":\"https:\\\/\\\/www.instrument-solutions.com\\\/wp-content\\\/uploads\\\/Knauer-logo-250px.png\",\"contentUrl\":\"https:\\\/\\\/www.instrument-solutions.com\\\/wp-content\\\/uploads\\\/Knauer-logo-250px.png\"},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/www.instrument-solutions.com\\\/en\\\/technology\\\/chromatography\\\/gpc-sec-chromatography#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\\\/\\\/www.instrument-solutions.com\\\/en\\\/home\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Technology\",\"item\":\"https:\\\/\\\/www.instrument-solutions.com\\\/en\\\/technology\"},{\"@type\":\"ListItem\",\"position\":3,\"name\":\"Chromatography\",\"item\":\"https:\\\/\\\/www.instrument-solutions.com\\\/en\\\/technology\\\/chromatography\"},{\"@type\":\"ListItem\",\"position\":4,\"name\":\"GPC-SEC chromatography\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/www.instrument-solutions.com\\\/en#website\",\"url\":\"https:\\\/\\\/www.instrument-solutions.com\\\/en\",\"name\":\"Instrument Solutions\",\"description\":\"elementanalyse | chromatografie | elektronische sensing\",\"publisher\":{\"@id\":\"https:\\\/\\\/www.instrument-solutions.com\\\/en#organization\"},\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/www.instrument-solutions.com\\\/en?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"},{\"@type\":\"Organization\",\"@id\":\"https:\\\/\\\/www.instrument-solutions.com\\\/en#organization\",\"name\":\"Instrument Solutions Benelux B.V.\",\"url\":\"https:\\\/\\\/www.instrument-solutions.com\\\/en\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\\\/\\\/www.instrument-solutions.com\\\/en#\\\/schema\\\/logo\\\/image\\\/\",\"url\":\"https:\\\/\\\/www.instrument-solutions.com\\\/wp-content\\\/uploads\\\/logo-instrument-solutions-transparant.png\",\"contentUrl\":\"https:\\\/\\\/www.instrument-solutions.com\\\/wp-content\\\/uploads\\\/logo-instrument-solutions-transparant.png\",\"width\":728,\"height\":325,\"caption\":\"Instrument Solutions Benelux B.V.\"},\"image\":{\"@id\":\"https:\\\/\\\/www.instrument-solutions.com\\\/en#\\\/schema\\\/logo\\\/image\\\/\"}}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"GPC-SEC chromatography - Instrument Solutions","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.instrument-solutions.com\/en\/technology\/chromatography\/gpc-sec-chromatography","og_locale":"en_US","og_type":"article","og_title":"GPC-SEC chromatography - Instrument Solutions","og_url":"https:\/\/www.instrument-solutions.com\/en\/technology\/chromatography\/gpc-sec-chromatography","og_site_name":"Instrument Solutions","article_modified_time":"2025-10-22T09:32:24+00:00","og_image":[{"url":"https:\/\/www.instrument-solutions.com\/wp-content\/uploads\/Knauer-logo-250px.png","type":"","width":"","height":""}],"twitter_misc":{"Est. reading time":"19 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/www.instrument-solutions.com\/en\/technology\/chromatography\/gpc-sec-chromatography","url":"https:\/\/www.instrument-solutions.com\/en\/technology\/chromatography\/gpc-sec-chromatography","name":"GPC-SEC chromatography - Instrument Solutions","isPartOf":{"@id":"https:\/\/www.instrument-solutions.com\/en#website"},"primaryImageOfPage":{"@id":"https:\/\/www.instrument-solutions.com\/en\/technology\/chromatography\/gpc-sec-chromatography#primaryimage"},"image":{"@id":"https:\/\/www.instrument-solutions.com\/en\/technology\/chromatography\/gpc-sec-chromatography#primaryimage"},"thumbnailUrl":"https:\/\/www.instrument-solutions.com\/wp-content\/uploads\/Knauer-logo-250px.png","datePublished":"2025-10-22T09:32:04+00:00","dateModified":"2025-10-22T09:32:24+00:00","breadcrumb":{"@id":"https:\/\/www.instrument-solutions.com\/en\/technology\/chromatography\/gpc-sec-chromatography#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/www.instrument-solutions.com\/en\/technology\/chromatography\/gpc-sec-chromatography"]}]},{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/www.instrument-solutions.com\/en\/technology\/chromatography\/gpc-sec-chromatography#primaryimage","url":"https:\/\/www.instrument-solutions.com\/wp-content\/uploads\/Knauer-logo-250px.png","contentUrl":"https:\/\/www.instrument-solutions.com\/wp-content\/uploads\/Knauer-logo-250px.png"},{"@type":"BreadcrumbList","@id":"https:\/\/www.instrument-solutions.com\/en\/technology\/chromatography\/gpc-sec-chromatography#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/www.instrument-solutions.com\/en\/home"},{"@type":"ListItem","position":2,"name":"Technology","item":"https:\/\/www.instrument-solutions.com\/en\/technology"},{"@type":"ListItem","position":3,"name":"Chromatography","item":"https:\/\/www.instrument-solutions.com\/en\/technology\/chromatography"},{"@type":"ListItem","position":4,"name":"GPC-SEC chromatography"}]},{"@type":"WebSite","@id":"https:\/\/www.instrument-solutions.com\/en#website","url":"https:\/\/www.instrument-solutions.com\/en","name":"Instrument Solutions","description":"elementanalyse | chromatografie | elektronische sensing","publisher":{"@id":"https:\/\/www.instrument-solutions.com\/en#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/www.instrument-solutions.com\/en?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"},{"@type":"Organization","@id":"https:\/\/www.instrument-solutions.com\/en#organization","name":"Instrument Solutions Benelux B.V.","url":"https:\/\/www.instrument-solutions.com\/en","logo":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/www.instrument-solutions.com\/en#\/schema\/logo\/image\/","url":"https:\/\/www.instrument-solutions.com\/wp-content\/uploads\/logo-instrument-solutions-transparant.png","contentUrl":"https:\/\/www.instrument-solutions.com\/wp-content\/uploads\/logo-instrument-solutions-transparant.png","width":728,"height":325,"caption":"Instrument Solutions Benelux B.V."},"image":{"@id":"https:\/\/www.instrument-solutions.com\/en#\/schema\/logo\/image\/"}}]}},"jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/www.instrument-solutions.com\/en\/wp-json\/wp\/v2\/pages\/47614","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.instrument-solutions.com\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.instrument-solutions.com\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.instrument-solutions.com\/en\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.instrument-solutions.com\/en\/wp-json\/wp\/v2\/comments?post=47614"}],"version-history":[{"count":6,"href":"https:\/\/www.instrument-solutions.com\/en\/wp-json\/wp\/v2\/pages\/47614\/revisions"}],"predecessor-version":[{"id":47621,"href":"https:\/\/www.instrument-solutions.com\/en\/wp-json\/wp\/v2\/pages\/47614\/revisions\/47621"}],"up":[{"embeddable":true,"href":"https:\/\/www.instrument-solutions.com\/en\/wp-json\/wp\/v2\/pages\/8909"}],"wp:attachment":[{"href":"https:\/\/www.instrument-solutions.com\/en\/wp-json\/wp\/v2\/media?parent=47614"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}